Relationship Between Aging and Renal High-Affinity Sodium-Dependent Dicarboxylate Cotransporter-3 Expression Characterized With Antifusion Protein Antibody
Identifieur interne : 001093 ( Main/Exploration ); précédent : 001092; suivant : 001094Relationship Between Aging and Renal High-Affinity Sodium-Dependent Dicarboxylate Cotransporter-3 Expression Characterized With Antifusion Protein Antibody
Auteurs : Jianzhong Wang [République populaire de Chine] ; Xiangmei Chen [République populaire de Chine] ; Hanyu Zhu [République populaire de Chine] ; Lixia Peng [République populaire de Chine] ; Quan Hong [République populaire de Chine]Source :
- The Journals of Gerontology Series A: Biological Sciences and Medical Sciences [ 1079-5006 ] ; 2003.
English descriptors
- Teeft :
- Accessible surface, Adult group, Amino acids, Antigen epitopes, Basolateral, Basolateral membrane, Beijing, Biol chem, Body temperature, Brush border, Caloric restriction, Calorie restriction, Child group, Chromatography columns, Coli, Color development, Color reaction, Dicarboxylate, Dicarboxylate cotransporter, Dicarboxylate transporter, Dnastar software, Ecor, Epithelial cells, Equal volume, Experimental animal center, Expression level, Fusion protein, Fusion protein antigen, General hospital, Goat antirabbit, Group kidney, Group kidney tissue, Higher antigen index number, Higher levels, Human kidneys, Immunogen peptide, Immunohistochemistry, Independent experiments, Indy gene, Intracellular domain, Iptg, Kidney, Kidney center, Kidney sections, Kidney tissue, Kidney tissues, Krebs cycle, Liberation army, Life span, Lipid peroxide production, Membrane, Metabolic acidosis, Mitochondrial matrix, Mrna, Nadc, Natural science foundation, Nitrocellulose membrane, Normal goat serum, Normal humans, Northern blots, Other groups, Plasmid, Pliable structure, Polyclonal antibody, Present study, Primer, Protein abundance, Protein expression, Protein samples, Proximal, Proximal tubule, Rat, Ratio graph, Recombinant, Recombined plasmid, Renal, Rhesus monkeys, Rnazol reagent, Room temperature, Sequencing result, Total protein, Tubule, Wang, Western blot assay, Wistar, Wistar rats, Xiangmei chen.
Abstract
Sodium-dependent dicarboxylate cotransporter (NaDC), which is responsible for the transportation of intermediates of the Krebs cycle, has been implicated in extending the life span of drosophila. In the present study, we cloned an intracellular domain segment of human kidney NaDC-3, which is 75% identical to that of the rat, constructed a polyclonal antibody against fusion protein of glutathione S-transferase (GST)-NaDC-3, and detected its renal expression changes with aging in both Wistar rats and normal humans. Western blot and immunohistochemistry confirmed the specificity of the antibody, and its location was found to be on the basolateral membrane of the renal proximal tubule. In addition, Western and Northern blots showed that NaDC-3 in kidneys significantly increased with age in Wistar rats. In healthy humans, renal NaDC-3 abundance also increased with age. Our results demonstrated that NaDC-3 expression was increased in aged Wistar rats and aged people, indicating that NaDC-3 may have a role in the process of kidney aging.
Url:
DOI: 10.1093/gerona/58.10.B879
Affiliations:
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unit="vol">58</biblScope><biblScope unit="issue">10</biblScope><biblScope unit="page" from="B879">B879</biblScope><biblScope unit="page" to="B888">B888</biblScope></imprint><idno type="ISSN">1079-5006</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1079-5006</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Accessible surface</term><term>Adult group</term><term>Amino acids</term><term>Antigen epitopes</term><term>Basolateral</term><term>Basolateral membrane</term><term>Beijing</term><term>Biol chem</term><term>Body temperature</term><term>Brush border</term><term>Caloric restriction</term><term>Calorie restriction</term><term>Child group</term><term>Chromatography columns</term><term>Coli</term><term>Color development</term><term>Color reaction</term><term>Dicarboxylate</term><term>Dicarboxylate cotransporter</term><term>Dicarboxylate transporter</term><term>Dnastar software</term><term>Ecor</term><term>Epithelial 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serum</term><term>Normal humans</term><term>Northern blots</term><term>Other groups</term><term>Plasmid</term><term>Pliable structure</term><term>Polyclonal antibody</term><term>Present study</term><term>Primer</term><term>Protein abundance</term><term>Protein expression</term><term>Protein samples</term><term>Proximal</term><term>Proximal tubule</term><term>Rat</term><term>Ratio graph</term><term>Recombinant</term><term>Recombined plasmid</term><term>Renal</term><term>Rhesus monkeys</term><term>Rnazol reagent</term><term>Room temperature</term><term>Sequencing result</term><term>Total protein</term><term>Tubule</term><term>Wang</term><term>Western blot assay</term><term>Wistar</term><term>Wistar rats</term><term>Xiangmei chen</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Sodium-dependent dicarboxylate cotransporter (NaDC), which is responsible for the transportation of intermediates of the Krebs cycle, has been implicated in extending the life span of drosophila. In the present study, we cloned an intracellular domain segment of human kidney NaDC-3, which is 75% identical to that of the rat, constructed a polyclonal antibody against fusion protein of glutathione S-transferase (GST)-NaDC-3, and detected its renal expression changes with aging in both Wistar rats and normal humans. Western blot and immunohistochemistry confirmed the specificity of the antibody, and its location was found to be on the basolateral membrane of the renal proximal tubule. In addition, Western and Northern blots showed that NaDC-3 in kidneys significantly increased with age in Wistar rats. In healthy humans, renal NaDC-3 abundance also increased with age. Our results demonstrated that NaDC-3 expression was increased in aged Wistar rats and aged people, indicating that NaDC-3 may have a role in the process of kidney aging.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Wang, Jianzhong" sort="Wang, Jianzhong" uniqKey="Wang J" first="Jianzhong" last="Wang">Jianzhong Wang</name></noRegion><name sortKey="Chen, Xiangmei" sort="Chen, Xiangmei" uniqKey="Chen X" first="Xiangmei" last="Chen">Xiangmei Chen</name><name sortKey="Hong, Quan" sort="Hong, Quan" uniqKey="Hong Q" first="Quan" last="Hong">Quan Hong</name><name sortKey="Peng, Lixia" sort="Peng, Lixia" uniqKey="Peng L" first="Lixia" last="Peng">Lixia Peng</name><name sortKey="Zhu, Hanyu" sort="Zhu, Hanyu" uniqKey="Zhu H" first="Hanyu" last="Zhu">Hanyu Zhu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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